Production of tailor-made fatty acids from crude glycerol at low pH by Yarrowia lipolytica
Adam Dobrowolski , Katarzyna Drzymała , Paweł Mituła , Aleksandra Mirończuk
AbstractNowadays, single cell oil (SCO) can play two distinct roles, first as a supplier of functional oils, and second as a feedstock for the biodiesel industry. These two distinct functions require a different fatty acids (FA) profile in the lipid pool. Moreover, to exploit their potential for industrialization, it is necessary to employ a low-cost substrate. Crude glycerol is the main side-product of biodiesel production. This renewable feedstock is one of Yarrowia lipolytica favorable substrates. In this study we improved polyunsaturated fatty acids (PUFA) synthesis by overexpression of the glycerol phosphate acyltransferase gene (SCT1). Here, we established a method to alter the quantity and FA composition of SCO. The engineered strain showed a 10-fold improvement (>20%) in linoleic acid synthesis (C18:2) in a shake-flask experiment. In a fermenter study co-overexpression of glycerol kinase (GUT1) and SCT1 allowed for 3-fold improvement in C18:2 synthesis from crude glycerol and at low pH.
|Journal series||Bioresource Technology, ISSN 0960-8524, e-ISSN 1873-2976, (N/A 140 pkt)|
|Keywords in English||Yarrowia lipolytica, Polyunsaturated fatty acid, PUFA, Glycerol phosphate acyltransferase, SCT1|
|ASJC Classification||; ; ; ;|
|Score||= 140.0, 14-09-2020, ArticleFromJournal|
|Publication indicators||= 0; : 2018 = 1.824; : 2018 = 6.669 (2) - 2018=6.589 (5)|
* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.