Expression and Complex Formation of MMP9, MMP2, NGAL, and TIMP1 in Porcine Myocardium but Not in Skeletal Muscles in Male Pigs with Tachycardia-Induced Systolic Heart Failure
Liliana Kiczak , Alicja Tomaszek , Jacek Bania , Urszula Pasławska , Maciej Zacharski , Agnieszka Noszczyk-Nowak , Adrian Janiszewski , Piotr Skrzypczak , Hossein Ardehali , Ewa Anita Jankowska , Piotr Ponikowski
AbstractMatrix metalloproteinases (MMPs) are involved in the remodeling of extracellular matrix in various tissues. Their functioning could be related to the formation of complexes, containing MMP9, MMP2, tissue inhibitor of metalloproteinases type 1 (TIMP1), and neutrophil gelatinase-associated lipocalin (NGAL). Such complexes have not been investigated in eithermyocardial or skeletal muscles. We examined 20 male pigs with heart failure (HF), and 5 sham-operated animals. There were no differences in the mRNA expression of MMP9, MMP2, TIMP1, and NGAL between diseased and healthy animals, in either left ventricle (LV) myocardium or skeletal muscles. In LV from both diseased and healthy animals, in nonreducing and nondenaturing conditions, we demonstrated the presence of high molecular weight (HMW) complexes (130, 170, and 220 kDa) containing MMP9, TIMP1, and NGAL (also MMP2 in 220 kDa complex) without proteolytic activity, and a proteolytically active 115 kDa MMP9 form together with 72 and 68 kDa bands (proMMP2 and MMP2). Proteolytically active bands were also spontaneously released from HMW complexes. In skeletal muscles from both diseased and healthy animals, in nonreducing and nondenaturing conditions, we found no HMW complexes, and proteolytic activity was associated with the presence of 72 and 68 kDa bands (proMMP2 and MMP2).
|Journal series||BioMed Research International, [Journal of Biomedicine and Biotechnology], ISSN 2314-6133, e-ISSN 2314-6141, [2314-6141], (A 30 pkt)|
|ASJC Classification||; ; ; ; ; ; ;|
|Score|| = 30.0, 14-07-2020, ArticleFromJournal|
= 30.0, 14-07-2020, ArticleFromJournal
|Publication indicators||= 16; = 15; : 2014 = 0.716; : 2013 = 2.706 (2) - 2013=2.75 (5)|
|Citation count*||18 (2020-09-16)|
* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.