Application of bone marrow and adipose-derived mesenchymal stem cells for testing the biocompatibility of metal-based biomaterials functionalized with ascorbic acid
Krzysztof Marycz , Agnieszka Śmieszek , Jakub Grzesiak , Anna Donesz-Sikorska , Justyna Krzak-Ros
AbstractIn this study, metal-based biomaterials were functionalized with ascorbic acid (LAA). Two types of substrates were used: austenitic steel 316L and titanium Ti6Al4V. Coatings were prepared with the sol-gel method and applied on metal surfaces using the dip-coating technique. Ascorbic acid was delivered with SiO2-coating at concentrations of 0.1 and 0.4 M. The morphology of the surfaces and coatings was determined using scanning electron microscope (SEM), whereas their elemental composition by SEM-EDX. Immobilization of ascorbic acid in the coatings was confirmed with Raman spectroscopy. The biocompatibility of the materials obtained was tested in vitro using both bone marrow-and adipose-derived mesenchymal stem cells (BMMSC and ADMSC, respectively). Proliferation rate and morphology of cells cultured in the presence of designed biomaterials were monitored after 24, 48, 120 and 168 h of propagation. The results obtained indicated that silica coatings doped with 0.4 M LAA had a positive effect on the proliferation rate of investigated cells, and in some cases on the growth pattern of culture.
|Journal series||Biomedical Materials, ISSN 1748-6041, (A 30 pkt)|
|ASJC Classification||; ;|
|Score|| = 30.0, 02-09-2020, ArticleFromJournal|
= 30.0, 02-09-2020, ArticleFromJournal
|Publication indicators||= 39; = 44; : 2013 = 0.826; : 2013 = 2.922 (2) - 2013=2.898 (5)|
|Citation count*||50 (2020-09-24)|
* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.