Spectroscopic and biochemical characteristics of flax transgenic callus cultures producing PHB
Magdalena Wróbel-Kwiatkowska , Kamil Kostyn , Lucyna Dymińska , Jerzy Hanuza , Adam Kurzawa , Magdalena Żuk , Waldemar Rymowicz
AbstractLinum usitatissimum L. is a plant used by human since ancient times. Presently fax has both industrial and nutritional signifcance. The programmes of restoration of fax cultivation and processing are implemented to renew the importance of this plant for agriculture and economy. Genetic engineering methods and techniques of plant tissue cultures can answer this requirement, because they give the opportunity for obtaining of new plants and novel application of them. In the present study the impact of genetic modifcation of fax plants on the ability of morphogenesis was investigated. The subject of this research remain genetically modifed fax plants enriched in poly-β-hydroxybutyrate (PHB), interesting biodegradable compound showing bioactive properties and acting as anti-infammatory and anti-microbial factor. Another aim of this study was to characterize chemical and structural properties of callus derived from the engineered plants in comparison to the data obtained for non-transgenic, control callus cultures. The presence of PHB was confrmed by FTIR technique and it was suggested that this polymer may have impact on about 20% higher biomass growth observed for transgenic callus cultures in comparison to control cultures. The level of phenolic compounds was not signifcantly changed in the transgenic calluses when compared to wild-type cultures. The extracellular matrix (EMC) was noticed for the surface of genetically modifed calluses, such structure was not visible for calluses derived from the control cultures. The cellulose in plant cell walls of transgenic calluses was structurally changed, it was not as organized as in control callus, resulting in lower crystallinity index values.
|Journal series||Plant Cell Tissue and Organ Culture, [Plant Cell, Tissue and Organ Culture], ISSN 0167-6857, e-ISSN 1573-5044, (N/A 100 pkt)|
|Publication size in sheets||0.5|
|Keywords in English||Flax, Callus, Polyhydroxybutyrate (PHB), Fourier-transform infrared spectroscopy (FTIR), Scanning electron microscopy (SEM)|
|License||Journal (articles only); published final; ; with publication|
|Score||= 100.0, 20-06-2020, ArticleFromJournal|
|Publication indicators||= 0; : 2017 = 1.122; : 2018 = 2.2 (2) - 2018=2.232 (5)|
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