Effects of the Inclusion of Equex STM into Tris-Based Extender on the Motility of Dog Spermatozoa Incubated at 5 degrees C
Wojciech Niżański , M. Klimowicz , Agnieszka Partyka , M. Savic , A. Dubiel
AbstractThe aim of this study was to estimate the effects of Equex STM on sperm motion characteristics in chilled dog semen extended in Tris-based diluent. Thirty-two ejaculates were collected from 12 proven German shepherd stud dogs. The sperm-rich fractions were diluted in Tris-based extender with 1% (v/v) Equex STM (sample A) and in Tris-based extender with no addition of detergent (sample B). The extended semen was incubated for 240 h at 5 degrees C and the motility parameters were evaluated by CASA system at 24-h intervals. Addition of Equex STM to Tris-based extender led to an initial activation of motion activity of spermatozoa, followed by a rapid decrease, shortening the lifespan of spermatozoa incubated at 5 degrees C. Computer-assisted sperm analysis clearly showed that Equex STM-induced changes of sperm motion characteristics resemble the hyperactivation (HA) of spermatozoa associated with the capacitation process.
|Journal series||Reproduction in Domestic Animals, ISSN 0936-6768, (A 30 pkt)|
|Publication size in sheets||0.3|
|ASJC Classification||; ;|
|Publication indicators||= 21; = 23; : 2009 = 0.895; : 2009 = 1.606 (2) - 2009=1.789 (5)|
* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.