Comparative quantitative and qualitative analysis of mitochondria in chronic hepatitis B and C patients
Patryk Smoliński , Marcin Nowak , Barbara Dolińska-Krajewska
Background. Studies imply that mitochondria have a role in viral pathologies, but hardly any objective proof has been presented based on structural analysis. Objectives. In this study, changes in hepatocyte mitochondria in chronic hepatitis B (CHB) and C (CHC) patients were analyzed and compared with each other and with reference data. Material and Methods. Overall, 1200 hepatocyte mitochondria of CHB and CHC patients were analyzed. Mitochondria were assessed at x15,000 and x5000 magnification in a scanning electron microscope. All parameters were analyzed with MultiScane Base v 8.08 and statistically analyzed with Statistica PL v. 5.01 using the Student's t, χ2, and Pearson's linear correlation tests. Results. The average numbers of mitochondria seen in both groups did not differ (p = 0.76). A difference in the number of shape-changed organelles was especially noted in CHC rather than CHB (p = 0.00026). A significant difference was found in the occurrence of abnormally large mitochondria (p = 0.01). The average number of mitochondrial cristae varied in both groups, more in CHC than CHB (p = 0.0004). Special differences were noted in the length of the cristae (p = 0.0075), but no clear change was found in the intermembrane space diameter (p = 0.04). The average mitochondrion area significantly differed in both groups and larger values were noted in the CHB group (p = 0.0001 Conclusions. The mitochondrial structural changes are not homogenous in the HBV and HCV groups. Abnormalities were more prevalent in CHC patients and especially concerned shape changes, differences in mitochondrial length and width, and internal structural changes rather than the area or overall number of mitochondria. Assuming that the observed mitochondrial changes were related exclusively to viral pathogens, the changes in their structure revealed by electron microscopic analysis strongly imply that severe changes in function are induced more by HCV than HBV. © Copyright by Wroclaw Medical University.
|Journal series||Advances in Clinical and Experimental Medicine, ISSN 1899-5276, [1230-025X], (A 15 pkt)|
|Keywords in English||Analysis, HBV, HCV, Mitochondria, Viral hepatitis|
|Publication indicators||= 2; = 2; : 2009 = 0.1; : 2011 = 0.176 (2) - 2012=0.204 (5)|
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