Differences in cysteine peptidases-like activity in sera of patients with breast cancer
Ewa Kilar , Maciej Siewiński , Lidia Hirnle , Teresa Skiba , Krzysztof Goła̧b , Jakub Gburek , Marek Murawski , Anna Janocha
AbstractBACKGROUND: The key role in carcinogenesis with destruction of the extracellular matrix is played by proteases released by invasive cancer cells. Cysteine peptidases, such as cathepsin B and L, take an important role in cancer progression and metastasis. OBJECTIVES: Cysteine peptidase-like activity (CPA) in sera of patients with breast cancer at different stages of disease and the influence of genetic predisposition associated with BRCA-1 gene mutations were analysed. METHODS: CPA in serum was determined with the spectrofluorometric technique using Z-Phe-Arg-AMC as a substrate. Determination was carried out in 111 breast cancer patients in comparison to a control group of 50 healthy subjects. RESULTS: The highest CPA was found in breast cancer patients with a hereditary predisposition bearing BRCA1 gene mutations, and the lowest activity was found in patients who had a tumour surgically removed and before adjuvant therapy. The differences in the activities between control group and cancer groups were statistically significant (p< 0.05), except from group of cancer patients in complete remission (p< 0.52). CONCLUSIONS: Serum CPA in patients with breast cancer differs depending on the cancer stage and treatment methods. Our study demonstrate the correlation between BRCA-1 gene mutations and the increased level of CPA.
|Journal series||Cancer Biomarkers, ISSN 1574-0153, e-ISSN 1875-8592, (N/A 70 pkt)|
|Publication size in sheets||0.5|
|Keywords in English||Breast cancer, serum biomarkers, cysteine peptidases-like activity, cathepsins|
|ASJC Classification||; ; ;|
|Score||= 70.0, 15-09-2020, ArticleFromJournal|
|Publication indicators||= 0; = 0; : 2018 = 0.57; : 2019 = 3.436 (2) - 2019=3.089 (5)|
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