Application of DNA markers linked to the potato H1 gene conferring resistance to pathotype Ro1 of Globodera rostochiensis
Renata Galek , M. Rurek , Walter S. de Jong , Grzegorz Pietkiewicz , Halina Augustyniak , Ewa Sawicka-Sienkiewicz
AbstractNinety-one potato genotypes (cultivars and breeding lines) selected as resistant or susceptible to pathotype Ro1 of Globodera rostochiensis were screened for the presence of two PCR markers, 0.14 and 0.76 kb in length. Both PCR markers were linked with the H1 gene, located at the distal end of the long arm of chromosome V, and were present in 88 to 100% of the resistant cultivars and breeding lines. The 0.76 kb PCR marker was detected in all resistant genotypes and in approximately 86% of susceptible breeding lines as well as in all susceptible cultivars. The 0.14 kb marker was detected in 88% of resistant breeding lines and in 94% of resistant cultivars. Most of the susceptible genotypes tested (91% of cultivars, but only 50% of breeding lines) did not show the presence of the 0.14 kb marker. We conclude that the 0.14 kb H1 marker is likely to be useful for the proper selection of potato genotypes resistant to the Ro1 pathotype of G. rostochiensis.
|Journal series||Journal of Applied Genetics, ISSN 1234-1983, e-ISSN 2190-3883, (A 20 pkt)|
|Publication size in sheets||0.5|
|Keywords in English||Globodera rostochiensis; Marker; Pathotype Ro1; PCR|
|Publication indicators||= 17; = 11; : 2011 = 1.042; : 2011 = 1.664 (2) - 2011=1.749 (5)|
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