UPLC-PDA-Q/TOF-MS profiling of phenolic and carotenoid compounds and their influence on anticholinergic potential for AChE and BuChE inhibition and on-line antioxidant activity of selected Hippophaë rhamnoides L. cultivars
Karolina Tkacz , Aneta Wojdyło , Igor Turkiewicz , Federico Ferreres , Diego A. Moreno , Paulina Nowicka
AbstractThis study aimed to identify by UPLC-PDA-Q/TOF-MS and quantify by UPLC-PDA phenolic compounds (26 flavonols and 2 phenolic acids) and carotenoids (16) from berries of different cultivars of Hippophaë rhamnoides and determine correlations between these variables and in vitro anticholinergic and on-line antioxidant potential. Isorhamnetin derivatives presented over 65% of total flavonols, but quercetin and kaempferol derivatives were also determined. Carotenes accounted for 19 to 47%, xanthophylls 16 to 81% of total carotenoids. Pearson’s correlations between AChE and BuChE inhibition and phenolic acid content were low (r = 0.388 and 0.355), moderate for carotenoids (0.504 and 0.437) and high for flavonols (0.851 and 0.614). The PCA biplot showed the highest correlation between anticholinergic activity and all-trans-ß-cryptoxanthin, quercetin-3-O-glucoside, isorhamnetin-3-O-(2-rhamnosyl)glucoside, kaempferol-3-O-hexoside-7-O-rhamnoside, isorhamnetin-3-O-(6-rhamnosyl)hexoside, isorhamnetin-3-O-rutinoside, and isorhamnetin-3-O-glucoside concentrations. The results obtained can be used to identify sea buckthorn cultivars, develop crops and production, and design functional products rich in flavonols and carotenoids with anticholinergic properties.
|Journal series||Food Chemistry, ISSN 0308-8146, e-ISSN 1873-7072, (N/A 200 pkt)|
|Publication size in sheets||0.5|
|Keywords in English||Sea buckthorn, Flavonols, Phenolic acids, Carotenoids, Anticholinergic, AChE, BuChE, ABTS on-line, PCA|
|ASJC Classification||; ;|
|Score||= 200.0, 21-04-2021, ArticleFromJournal|
|Publication indicators||= 9; = 8; : 2018 = 2.17; : 2019 = 6.306 (2) - 2019=6.219 (5)|
|Citation count*||14 (2021-05-12)|
* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.