Identification of natural mutations responsible for altered infection phenotypes of Salmonella enterica clinical isolates using cell line infection screens
Rafał Kolenda , Michał Burdukiewicz , Marcjanna Wimonć , Adrianna Aleksandrowicz , Aamir Ali , Istvan Szabo , Karsten Tedin , Josefine Bartholdson Scott , Derek Pickard , Peter Schierack
AbstractThe initial steps ofSalmonellapathogenesis involve adhesion to and in-vasion into host epithelial cells. While well-studied forSalmonella entericaserovar Ty-phimurium, the factors contributing to this process in other, host-adapted serovarsremains unexplored. Here, we screened clinical isolates of serovars Gallinarum, Dub-lin, Choleraesuis, Typhimurium, and Enteritidis for adhesion to and invasion into in-testinal epithelial cell lines of human, porcine, and chicken origins. Thirty isolateswith altered infectivity were used for genomic analyses, and 14 genes and novelmutations associated with high or low infectivity were identified. The functions ofcandidategenes included virulence gene expression regulation and cell wall or mem-brane synthesis and components. The role of several of these genes inSalmonellaadhe-sion to and invasion into cells has not previously been investigated. The genesdksA(encoding a stringent response regulator) andsanA(encoding a vancomycin high-temperature exclusion protein) were selected for further analyses, and we confirmedtheir roles in adhesion to and invasion into host cells. Furthermore, transcriptomic analy-ses were performed forS. Enteritidis andS. Typhimurium, with two highly infective andtwo marginally infective isolates for each serovar. Expression profiles for the isolates withaltered infection phenotypes revealed the importance of type 3 secretion system expres-sion levels in the determination of an isolate’s infection phenotype. Taken together,these data indicate a new role in cell host infection for genes or gene variants previ-ously not associated with adhesion to and invasion into the epithelial cells.
|Journal series||Applied and Environmental Microbiology, ISSN 0099-2240, e-ISSN 1098-5336, (N/A 100 pkt)|
|Publication size in sheets||0.8|
|Keywords in English||Salmonella, adhesion, invasion, infection, clinical isolates, epithelial cells,sanA,dksA, genomics, transcriptomics, host cell invasion|
|ASJC Classification||; ; ;|
|License||Journal (articles only); published final; ; after publication|
|Score||= 100.0, 28-04-2021, ArticleFromJournal|
|Publication indicators||= 0; = 0; : 2018 = 1.272; : 2019 = 4.016 (2) - 2019=4.597 (5)|
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