Hydroxylation of DHEA, androstenediol and epiandrosterone by Mortierella isabellina AM212. Evidence indicating that both constitutive and inducible hydroxylases catalyze 7α- as well as 7β-hydroxylations of 5-ene substrates
Teresa Kołek , Natalia Milecka-Tronina , Alina Świzdor , Anna Panek , Agata Białońska
AbstractThe course of transformation of DHEA, androstenediol and epiandrosterone in Mortierella isabellina AM212 culture was investigated. The mentioned substrates underwent effective hydroxylation; 5-ene substrates - DHEA and androstenediol - were transformed into a mixture of 7α- and 7β- allyl alcohols, while epiandrosterone was converted into 7α- (mainly), 11α- and 9α- monohydroxy derivatives. Ketoconazole and cycloheximide inhibition studies suggest the presence of constitutive and substrate-induced hydroxylases in M. isabellina. On the basis of time course analysis of the hydroxylation of DHEA and androstenediol, the oxidation of allyl C 7-H α and C 7-H β bonds by the same enzyme is a reasonable assumption.
|Journal series||Organic & Biomolecular Chemistry, [Organic and Biomolecular Chemistry], ISSN 1477-0520, e-ISSN 1477-0539, (A 35 pkt)|
|Publication size in sheets||0.5|
|ASJC Classification||; ;|
|Publication indicators||= 23; = 18; : 2011 = 0.967; : 2011 = 3.696 (2) - 2011=3.652 (5)|
* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.