Long-term stiripentol administration, an anticonvulsant drug, does not impair sperm parameters in rats
Agnieszka Matuszewska , Beata Nowak , Adam Szeląg , Anna Merwid-Ląd , Agnieszka Partyka , Tomasz Tomkalski , Marzenna Podhorska-Okołów , Aleksandra Piotrowska , Joanna Kwiatkowska , Wojciech Niżański
AbstractIn this study, we investigated the influence of long‐term administration of stiripentol on sex hormones and semen quality in young Wistar rats. Investigated animals received for 6 months either stiripentol or saline solution. After one month, stiripentol increased temporarily serum level of testosterone (p < 0.05) and FSH (p < 0.01). However, after 6 months levels of testosterone, FSH, LH, prolactin and SHBG were comparable in both groups. After 6 months, semen analysis did not reveal differences in sperm concentration, total sperm count and sperm motility between groups. However, stiripentol increased the rate of head defect (p < 0.001) and midpiece abnormalities (p < 0.05). Flow cytometry revealed higher percentage of live cells without lipid peroxidation (p < 0.00001) and higher percentage of live spermatozoa with intact acrosomes (p < 0.000001) in rats receiving stiripentol. There was no significant difference between groups in sperm mitochondrial activity and DNA fragmentation index. However, percentage of high DNA stainability cells was increased in stiripentol group (p < 0.001). The data showed that stiripentol does not cause obvious disturbances in young rat's semen. Detected changes in semen morphology and chromatin structure need further explanation, and their influence on rat's fertility should be evaluated.
|Journal series||Andrologia, ISSN 0303-4569, e-ISSN 1439-0272, (N/A 70 pkt)|
|Publication size in sheets||0.5|
|Keywords in English||anti‐epileptic drug; hormones; rat; semen; stiripentol|
|ASJC Classification||; ;|
|License||Journal (articles only); published final; ; with publication|
|Not used for evaluation||yes|
|Publication indicators||= 0; = 0; : 2018 = 0.84; : 2019 = 1.951 (2) - 2019=1.888 (5)|
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