Pre-Growth Culture Conditions Affect Type 1 Fimbriae-Dependent Adhesion of Salmonella
Beata Klasa , Anna Ewa Kędzierska , Krzysztof Grzymajło
AbstractAmong various fimbrial structures used by Salmonella enterica to colonize host tissues, type 1 fimbriae (T1F) are among the most extensively studied. Although some experiments have shown the importance of T1F in the initial stages of Salmonella infection, their exact role in the infection process is not fully known. We suggested that different outcomes of T1F investigations were due to the use of different pre-infection growth conditions for the induction of the T1F. We utilized qPCR, flow cytometry, and a wide range of adhesion assays to investigate Salmonella Choleraesuis and Salmonella Typhimurium adhesion in the context of T1F expression. We demonstrated that T1F expression was highly dependent on the pre-infection growth conditions. These growth conditions yielded T1F+ and T1F- populations of Salmonella and, therefore, could be a factor influencing Salmonella-host cell interactions. We supported this conclusion by showing that increased levels of T1F expression directly correlated with higher levels of Salmonella adherence to the intestinal epithelial IPEC-J2 cell line.
|Journal series||International Journal of Molecular Sciences, ISSN 1422-0067, (N/A 140 pkt)|
|Keywords in English||type 1 fimbriae; Salmonella; adhesion; phase variations; infection; growth conditions; passages|
|ASJC Classification||; ; ; ; ; ; ;|
|License||Journal (articles only); author's original; ; after publication|
|Score||= 140.0, 28-04-2021, ArticleFromJournal|
|Publication indicators||= 1; = 1; : 2018 = 1.224; : 2018 = 4.183 (2) - 2018=4.331 (5)|
|Citation count*||1 (2021-05-06)|
* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.