Novel structurally related flavones augment cell death induced by rhsTRAIL
Joanna Bronikowska , Ewelina Szliszka , Edyta Kostrzewa-Susłow , Dagmara Jaworska , Zenon Czuba , Piotr Bednarski , Wojciech Król
AbstractTRAIL (tumor necrosis factor-related apoptosis-inducing ligand) was identified as a powerful activator of apoptosis in tumor cells and one of the most promising candidates for cancer therapy with no toxicity against normal tissues. However, many tumor cells are resistant to TRAIL-induced apoptosis. The aim of this work was to analyze the improvement of the anticancer effect of rhsTRAIL (recombinant human soluble TRAIL) by nine flavones: 5-Hydroxyflavone, 6-Hydroxyflavone, 7-Hydroxyflavone and their new synthetic derivatives 5-acetoxyflavone, 5-butyryloxyflavone, 6-acetoxyflavone, 6-butyryloxyflavone, 7-acetoxyflavone and 7-butyryloxyflavone. We examined the cytotoxic and apoptotic effects of rhsTRAIL enhanced by novel structurally-related flavones on SW480 and SW620 colon cancer cells using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide test, the lactate dehydrogenase assay and annexin V-FITC fluorescence staining. We observed a slight difference in the activities of the flavones that was dependent on their chemical structure. Our study indicates that all nine flavones significantly augment cell death by rhsTRAIL (cytotoxicity range 36.8 ± 1.7%–91.4 ± 1.7%; apoptosis increase of 33.0 ± 0.7%–78.5 ± 0.9%). Our study demonstrates the potential use of tested flavones in TRAIL-based anticancer therapy and prevention.
|Journal series||International Journal of Molecular Sciences, ISSN 1422-0067, (A 30 pkt)|
|Publication size in sheets||0.8|
|Keywords in English||Flavones; Cytotoxicity; Apoptosis; TRAIL; Cancer cells|
|ASJC Classification||; ; ; ; ; ; ;|
|Internal identifier||63364; PX-59523b4fd5dea00757f7616e|
|License||Journal (articles only); published final; ; with publication|
|Score||= 30.0, 09-09-2020, ArticleFromJournal|
|Publication indicators||= 3; = 3; : 2017 = 1.151; : 2017 = 3.687 (2) - 2017=3.878 (5)|
* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.