Effects of equine metabolic syndrome on inflammation and acute-phase markers in horses
Agnieszka Żak , Natalia Siwińska , S. Elzinga , V.D. Barker , Tadeusz Stefaniak , B.J. Schanbacher , N.J. Place , Artur Niedźwiedź , Amanda Adams
AbstractObesity and metabolic disorders are associated with systemic low-grade chronic inflammation, both in humans and animals. The aim of the study is to assess the effects of obesity and hyperinsulinemia on individual components of the acute-phase reaction in equine metabolic syndrome (EMS) horses. Eight mixed-breed EMS and six control, age-matched horses of both sexes were included in the study. Animals were classified as EMS or control based on the assessment of BCS, cresty neck score, and basal insulin >50 μU/mL and/or insulin responses to the oral sugar test (OST) >60 μU/mL. Peripheral venous blood was collected. The expression of proinflammatory cytokines, the concentration of circulating cytokines, and acute-phase proteins (serum amyloid A, C-reactive protein, haptoglobin, activin A, and procalcitonin) were measured. The data were analyzed using the Mann-Whitney test, whereas correlations were examined using Spearman's correlation coefficient. The tests were statistically significant if P ≤ 0.05. There were no differences in cytokine gene expression, circulating cytokine concentrations, or concentrations of acute-phase proteins between the EMS and the control groups. There was a strong correlation between the basal concentration of insulin and the serum concentrations of IL-6 (r = 0.71, P < 0.05). Activin A was positively correlated with post-OST insulin concentrations (r = 0.707, P = 0.05), indicating that this marker of inflammation could warrant further investigation in horses with EMS.
|Journal series||Domestic Animal Endocrinology, ISSN 0739-7240, e-ISSN 1879-0054, (N/A 70 pkt)|
|Keywords in English||Activin A, Acute-phase proteins, IL-6Insulin dysregulation, Obesity|
|ASJC Classification||; ;|
|License||Journal (articles only); published final; ; with publication|
|Score||= 70.0, 15-09-2020, ArticleFromJournal|
|Publication indicators||= 1; = 1; : 2018 = 0.955; : 2018 = 2.302 (2) - 2018=2.063 (5)|
|Citation count*||3 (2020-09-23)|
* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.